Echo E-waves analyzes PW Doppler acquisitions in the DICOM format from all major vendors. Acquisitions should follow standard echocardiographic principles.

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It is recommended to acquire multiple E-waves per subject. This is in order to have an adequate number of high quality E-waves for curve fitting, as well as being a prerequisite for being able to study the response to variations in load (i.e. calculating M, the load independent index of filling). The program has the ability to automatically split continuous acquisitions from GE and Philips scanners into their constitutive heart beats, facilitating the analysis of several E-waves per subject.